On the second day, the size of each colony significantly increased compared to day one (P<0.001, paired t-test) for each individual condition ( i.e., control day 1 versus control day 2). On day one, one-way ANOVA shows no difference (P=0.09) in colony size for the 4 conditions tested (control 0.19☐.09 mm 2, 0.1 nM E2 0.20☐.08, fetal bovine serum (FBS) 0.23☐.11, FBS+ E2 0.24☐.12).
The diameter of each colony was computed using ImageJ and the surface area was calculated using Excel. Using bright field microscopy, images of five colonies in each condition were captured on day one and pictures of the same colonies were captured on day two. albicans colony sizes grown on yeast extract peptone dextrose (YEPD). In the present work, we investigated the effect of estrogen on C. E2 is also known to promote tissue growth, thus we hypothesize that E2 may influence C.
During sepsis, blood estrogen (E2) becomes elevated. albicans is a commensal fungus which under certain environmental cues shifts morphology from spores to filamentous and becomes invasive within the human body. albicans invades the mucosal barrier will greatly help developing new strategies for fighting candidiasis.Ĭ. albicans lower the intestinal defense mechanism. albicans induces NKCC1 internalization and may represent one mechanism by which C. Our preliminary experiments suggest that C. albicans, NKCC1 internalization was observed in some cells. Conversely, after 1- and 2-hours exposure to C. albicans for 15 and 30 min did not result in NKCC1 internalization as compared to 100 nM PMA. Fluorescent images were acquired using an inverted IX83 Olympus microscope equipped with a CCD camera. Coverslips were fixed in 1% paraformaldehyde for 30 min and mounted for fluorescence microscopy. MDCK were also exposed to 100 nM phorbol 12-myristate 13-acetate (PMA) for 15 min as a positive control for NKCC1 internalization. albicans, for 15, 30 min,1 and 2 h hours. On the day of the experiment, MDCK were exposed to 50,000 C. MDCK were cultured on coverslips in 6 well plate. To test our hypothesis, we utilized Madin-Darby Canine Kidney (MDCK) cells stably expressing eGFP-NKCC1. albicans, before invading intestinal cells, decreases fluid secretion by causing NKCC1 internalization. Previous work in the lab has demonstrated that the basolateral Na + -K + -2Cl – cotransporter 1 (NKCC1) plays a key role in the regulation of fluid secretion, and internalization of NKCC1 represents a potent pathway to blunt fluid secretion. albicans decreases the host defense mechanism prior to invading intestinal cells. In the gut, fluid secretion participates in the body defense by flushing microorganisms and by maintaining the surface mucus hydrated. albicans) is one of the most common human fungal pathogens, causing life threatening systemic infections in critically ill and immunocompromised patients. Here is the Schedule for SOURCE and the Graduate Symposium:ġ1:00 am-12:30 pm Morning Oral and Poster Presentationsġ:30- 3:00 pm Afternoon Oral and Poster PresentationsĬandida albicans (C. You can scroll down to view the titles and abstracts of all Oral and Poster presentations. After a lunch break, four additional Oral Presentation Sessions (O-5-PM through O-8-PM) and the Afternoon Poster Session (P-2-PM) will begin at 1:30 pm. Five Oral Presentation Sessions (O-1-AM through O-4-AM and O-9-AM) and the Morning Poster Session (P-1-AM) will take place starting at 11:00 am. The link for the 3:30 pm Awards Ceremony is just below. Click on the 10:30 am Opening and Welcome Session to access its recording. This page contains the final program for the 2022 SOURCE and the Graduate Academic Symposium, along with links to the Zoom Recordings of the Opening Session, the Oral Presentation Sessions, and the Awards Ceremony. SOURCE/Graduate Symposium 2022 (Final Program and Zoom Recording Links) SOURCE/Graduate Symposium 2022 (Final Program and Zoom Recording Links)
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